The wire myograph allows examination of small vessels (internal diameter 60μm-10mm) in terms of morphology and responsiveness to hormones and other agonists. The small vessels are mounted as ring preparations by threading them onto two stainless steel or tungsten wires and securing the wires to two supports.               One support is then attached to a micrometer, allowing control of vessel circumference. The other support is attached to a force transducer for measurement of tension development. The whole preparation is kept in a chamber with physiological salt solution at 37°C, bubbled with oxygen. Vessels maintained in wire myographs are viable for several hours.           Following mounting and equilibration, the passive length-tension relationships of the vessels are determined; a normalization procedure. During the actual experiments, the circumference of the vessels are kept constant and vessels are examined under isometric conditions. Compounds are added directly to the chamber and vessel tension is monitored. Furthermore, it is possible to compare vessels from patients or test groups with those of control, not only in terms of vessel reactivity to various compounds, but also in terms of morphology.            The following lists are a few of the established areas of investigation for wire myograph systems. Many more investigation possibilities for vascular and other smooth muscle may be added through the imagination of researchers such as yourself.             Basic tissue properties Vascular smooth muscle function Vascular endothelium function Length-tension relationships (also motorized) Wall tension & morphometric measurements Assessment of pharmacological reactivity             Dr. K. Dora, University of Bath, United Kingdom TIssues used Small and large arteries Veins Lymph vessels Lung and tracheal smooth muscle Urogenital, corpus cavernosum, bladder Vasoactive mechanisms Endothelium: role of endothelium-derived relaxing factor (NO), prostaglandins and endothelium-derived hyperpo-larizing factor (EDHF) Smooth muscle: role of calcium and potassium and other ion channels Perivascular and intramural nerves: role of endogenously released transmitters Pharmacology & pharmacotherapy Quantify the effect of treatment with contractile and relaxing agents Receptor studies, localization and characterization of receptors Affinity & efficacy studies of agonists & antagonists Drug studies efficiency & efficacy, drug discovery & safety pharmacology Physiological changes & pathology Hypertension Atherosclerosis Diabetes Ageing Ischemic heart disease & heart failure Tumors & angiogenesis Gastrointestinal & urogenital disease Pregnancy, preeclampsia Exercise physiology, muscular degenerative diseases Further possibilities Electrophysiological experiments Fluorescence measurements of intracellular ions and other substances                      Download Product System Brochure         See all Wire Myograph Systems            Products Software Accessories     ABOUT DMT PRODUCTS SOFTWARE About DMT Wire Myograph Systems Data Acquisition for Myograph   Contact DMT Pressure Myograph Systems Data Acquisition for Pressure Events Muscle Strip Myograph Systems Data Acquisition for TOBS Job Opportunities Tissue Organ Bath Systems   Myograph Users     News   Partners   Terms & Conditions Testimonials               ACCESSORIES SUPPORT & SERVICE DOWNLOAD   Automatic Buffer Filler System Onsite Traning Media Calibration Remote Support Brochures & spec. sheets Chamber Covers Service & Development Product Manuals Current Stimulator       Data Acquisition Systems     FlowMeter - 161FM Force/Pressure Transducers General Laboratory equipment Mounting Other Accessories Special parts

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