The Confocal Wire Myograph System - 120CW is specially designed to provide very close optical access to the mounted artery or tissue segment, thereby allowing high resolution images of fluorescent dyes or markers by laser scanning confocal microscopy (LSCM). This system elegantly combines LSCM with artery myography to allow simultaneous measurements of isometric force and fluorescence. A perfect example are studies that correlate isometric contractions in an isolated, mounted blood vessel and intracellular Ca2+ measurements within the vascular smooth muscle cells.
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»designed to provide very close optical access«
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The unique design of the chamber combines the precision and stability of conventional wire myographs with the added feature of precise Z-axis movement by a micrometer. This optimises the flexibility of using this system with different LSCMs and various high magnification objectives.
»For working distances smaller than 250 μm on inverted laser scanning confocal microscopes«
The bath design allows easy access for the high numerical aperture immersion objectives used on inverted microscopes and also direct immersion objectives used on standard upright microscopes. In addition, special mounting supports were specifically designed to allow precise vertical positioning of a mounted blood vessel or tissue ring directly above or on the chamber window. This permits the use of objectives with working distances smaller than 250 μm on an inverted LSCM. This may be advantageous when simultaneous electrophysiological measurements are collected from the top of the mounted tissue.
The chambers are supplied with custom covers for inverted or upright microscope systems. The covers include connections for suction, gassing or measurement electrodes (pH, NO, O2 tension).
»and vessel force/tension can be directly measured«
Compounds can be added directly to the chamber, and vessel force/tension can be directly measured while on the LSCM. Data acquired from the myograph (such as force and temperature) can be recorded continuously, either by using the user-defined analog outputs or the serial interface.
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Calcium transients within smooth muscle cells by
Dr. H. Nilsson, Aarhus University
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ACCESSORIES
| Mounting jaws - reverse
The myograph is supplied with jaws for inverted scopes but can be used with normal stainless steel jaws to mount small vessels on an upright system. For experimental work requiring electrical field stimulation a set of plastic jaws with attached platinum electrodes is available.
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